Molecular Biology Module

This laboratory exercise is composed of six parts to be completed over the course of a semester. The bacterial cultures used in this lab were obtained by last year's AP Biology class during their Sapelo Island trip. This lab reviews many of the molecular biology techniques that we need to get familiar with before the AP exam (see Chapter 20: Biotechnology in the AP Edition of Campbell and Reece’s Biology).

After completion of all parts you will prepare a written report summarizing your activities during this laboratory exercise. The lab report must be typewritten and should be 3-5 pages in length (single spaced). If you worked with a partner during the lab, do not write the lab report together...it must be entirely your own work (it will be a test grade). Include the following sections in your report:

Introduction:

What was the reason for carrying out this study? You might choose to focus your introduction on topics such as global biodiversity, or the role of bacteria in salt marshes, or any other framework that you think is relevant to the lab. There is no single right reason...you just need to choose something reasonable and present it well. Include at least 3 outside references (books, scientific papers), 2 of which must be different from the papers provided to you in lab. (Length of introduction: approximately 1 page).

Bacterial Sequencing:

Describe the laboratory methods you used to obtain a sequence of the 16S rRNA gene.

Sequence Analysis:

Describe what you learned about your organism and its relatives from the BLAST analysis at NCBI.

Lab Handouts:    
Laboratory Exercise (pdf) Laboratory Excercise (doc)  
Marine Bacteria Sequences (pdf) Marine Bacteria Sequences (doc)  
Molecular Genetics Table (pdf) Molecular Genetics Table (doc)  
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